Standard Calibration Buffer Solutions for pH/Conductivity/ORP/Turbidity etc..

The Intracellular pH Calibration Buffer Kit contains a range of pH calibration buffers (pH 4.5, 5.5, 6.5, and 7.5), as well as valinomycin and nigericin, which help equilibrate the pH inside and outside of cells. After using pHrodo™ Red AM or pHrodo™ Green AM and measuring fluorescence intensity under experimental conditions, the cells are then resuspended in one of the calibration buffers to which valinomycin and nigericin have been added, and the fluorescence intensity measured again. This is repeated with the remaining calibration buffers so that a standard curve can be created and used to pinpoint the intracellular pH associated with the experimental conditions.